- Simplemind map for sickle cell anemia how to#
- Simplemind map for sickle cell anemia software#
- Simplemind map for sickle cell anemia code#
Find out the chromosomal location of the gene that causes sickle cell anemia.Start your paragraph as a hypothesis as to which parts are most important, and write your discussion as a defense of your hypothesis. Print out your ClustalW results and attach a short paragraph discussing how Clustal W gives you a clue as to which part(s) of the Cytochrome C protein you would hypothesize are most important to its function (which is/are the same in all 3 organisms).Include answers from within today’s class.Workshop due as hardcopy when you arrive Thursday AM. Clink on the link, find the FASTA format and copy into the same file.Make sure the header is separate from the sequence. Copy the FASTA output for both species into a single text file.Enter the Accession Number (previous slide) and GO.Select to search the protein database from the dropdown menu.
Simplemind map for sickle cell anemia how to#
How to prepare the sequences for the MSA on ClustalW
Simplemind map for sickle cell anemia code#
Yeast protein number from structure database įASTA format for a protein sequence in single letter code Hemoglobin HBB1 >gi|4504349|ref|NP_000509.1| beta globin MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVAN ALAHKYHįASTA format for a protein sequence in single letter code >gi|1244762|gb|AAA98563.1| p53 tumor suppressor homolog MSQGTSPNSQETFNLLWDSLEQVTANEYTQIHERGVGYEYHEAEPDQTSLEISAYRIAQPDPYGRSESYD LLNPIINQIPAPMPIADTQNNPLVNHCPYEDMPVSSTPYSPHDHVQSPQPSVPSNIKYPGEYVFEMSFAQ PSKETKSTTWTYSEKLDKLYVRMATTCPVRFKTARPPPSGCQIRAMPIYMKPEHVQEVVKRCPNHATAKE HNEKHPAPLHIVRCEHKLAKYHEDKYSGRQSVLIPHEMPQAGSEWVVNLYQFMCLGSCVGGPNRRPIQLV FTLEKDNQVLGRRAVEVRICACPGRDRKADEKASLVSKPPSPKKNGFPQRSLVLTNDITKITPKKRKIDD ECFTLKVRGRENYEILCKLRDIMELAARIPEAERLLYKQERQAPIGRLTSLPSSSSNGSQDGSRSSTAFS TSDSSQVNSSQNNTQMVNGQVPHEEETPVTKCEPTENTIAQWLTKLGLQAYIDNFQQKGLHNMFQLDEFT LEDLQSMRIGTGHRNKIWKSLLDYRRLLSSGTESQALQHAASNASTLSVGSQNSYCPGFYEVTRYTYKHT ISYL.Dog protein accession number XP_532493.Human protein accession number AAA35732 (see next slide).Multiple sequence alignment for cytochrome C – mutation and conservation Summary DNA (mutated = changed) RNA (mutated) Protein (mutated) Remember: Mutation is not always “bad”! For example: Mutation → Evolution → An additional normal genome Tryptic digest: the protease trypsin cleaves C terminal to lysine and arginine. The early evidence that sickle cell anemia is caused by an amino acid change in hemoglobin. What is the quality of that difference with respect to R groups?.What is the amino acid difference in the two sequences?.How many nucleotides have changed in the codon in boldface?.ATG GTG CAC CTG ACT CCT GTG GAG AAG TCT GCC GTT ACT.
Simplemind map for sickle cell anemia software#
Website for Amino acid interactive Workshop What are the symptoms of sickle cell anemia? Ĭircle Triangle Square Bond Amino terminal Carboxy terminal.What class of biomolecules does hemoglobin belong to?.What do you already know about hemoglobin? Write down your answers or consolidate to print.If it’s review for you, use you intellect to hear it in a new way.Speak so that everyone from front to back can hear you.Ground Rules for Class Discussions and Workshops Sickle Cell Anemia An example of why: a change in protein can lead to disease a change in DNA can lead to a change in protein